Differential inactivation of nucleic acids by chemical modification
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United States of America Patent
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Dec 1, 1998
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Jan 19, 1996
filing date -
Jan 19, 1996
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Abstract
Disclosed herein is the method for dramatically reducing cytotoxicity of viral vectors such as Herpes simplex viral while retaining gene expression. The method of the invention virtually eliminates the concern of possible recombination during virus propagation and contamination of wild-type virus and virus stock. The invention comprises use of photochemical crosslinking causing differential inactivation of viruses.
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