Methods and compositions for cellular and metabolic engineering
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United States of America Patent
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Nov 17, 1998
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May 20, 1996
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Feb 17, 1994
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Abstract
The present invention is generally directed to the evolution of new metabolic pathways and the enhancement of bioprocessing through a process herein termed recursive sequence recombination. Recursive sequence recombination entails performing iterative cycles of recombination and screening or selection to 'evolve' individual genes, whole plasmids or viruses, multigene clusters, or even whole genomes. Such techniques do not require the extensive analysis and computation required by conventional methods for metabolic engineering.
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