Method and device for extraction and purification of DNA
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United States of America Patent
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Nov 10, 1998
Issued Date -
N/A
app pub date -
Nov 13, 1997
filing date -
Nov 4, 1994
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Abstract
A method for the extraction and purification of a plasmid DNA which comprises (1) collecting a culture medium containing a transformant into a first cartridge for the extraction and purification of the DNA, (2) subjecting said transformant to bacteriolysis and RNA degradation conditions, (3) recovering an extract containing solubilized DNA from said first cartridge, (4) transferring said recovered extract to a second cartridge for the extraction and purification of the DNA, and (5) absorbing, washing and eluting purified plasmid DNA from said second cartridge; and a device for the extraction and purification of a DNA which comprises a first cartridge for the extraction and purification of the DNA having at least a trap filter and a membrane filter, and a second cartridge for the extraction and purification of the DNA having at least a glass fiber filter, a glass powder layer and a membrane filter.
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