Conjugation of ligand to immobilized protein in organic solvent
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United States of America Patent
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Oct 6, 1998
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N/A
app pub date -
Nov 6, 1995
filing date -
Nov 6, 1995
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Expired
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Abstract
A new method for immobilization of small molecules on solid supports via a macromolecular spacer has been developed. A protein or another macromolecule is first immobilized in an aqueous medium, and the solid support is than washed with an organic solvent. The small molecule is coupled in an organic medium, followed by organic medium washes. The new solid phases are useful for affinity purifications, immunoassays and other binding assays, and for selection of binders by panning procedures.
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