Spectrophotometric methods for assaying total mercaptans, reduced glutathione (GSH) and mercaptans other than GSH in an aqueous medium reagents and kits for implementing same
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United States of America Patent
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Oct 6, 1998
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Aug 12, 1996
filing date -
Dec 20, 1991
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Abstract
According to the invention, the total mercaptans in an aqueous sample are assayed by means of the formation of a chromophoric substitution product between all the mercaptans present in the sample and a reagent corresponding to the general formula I: ##STR1## Reduced glutathione (GSH) is assayed in the form of a chromophoric thione formed specifically by alkalization from the substitution product formed above between GSH and the reagent. The concentration of mercaptans distinct from GSH is obtained by subtraction of the concentrations obtained in the preceding assays. Application to the assay of mercaptans and GSH, especially in the presence of other mercaptans, in particular in biological media.
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