Process for the identification or immunoassay of polypeptide antigens
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United States of America Patent
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Oct 6, 1998
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app pub date -
Dec 23, 1996
filing date -
Jan 18, 1996
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Abstract
Process for the identification or assay of a particular polypeptide antigen X in which an immunological determination of the presence of said polypeptide antigen X is carried out in a sample, characterized in that the sample is treated in vitro using at least one chemical reagent reacting on an epsilon -NH.sub.2 function of a lysine residue or on a terminal alpha -NH.sub.2 function of a peptide in order to carry out the linking of said reagent to said polypeptide antigen X and in this way to obtain a modified polypeptide antigen X, (polypeptide antigen Xm), the immunological determination of the presence of said polypeptide antigen X in said sample is then carried out using at least one antibody produced by immunization against a polypeptide antigen Y identical to the polypeptide antigen Xm or to an antigenic fragment of this antigen Xm.
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