Process for isolating and purifying nucleotide-activated sugars from biological sources
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United States of America Patent
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Sep 22, 1998
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N/A
app pub date -
Aug 30, 1995
filing date -
Sep 2, 1994
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Expired
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Abstract
The present invention relates to an improved process for isolating and purifying sugar nucleotides, in particular cytidine monophosphate-activated N-acetylneuraminic acid (CMP-Nana), from biological sources. The sugar nucleotides can be obtained from a sugar nucleotide-containing solution, which has been freed of proteins by alcoholic precipitation, by means of an improved column-chromatographic method which uses silica gel as the stationary phase, and at a purity level which requires only a subsequent desalting step to obtain a substantially pure product.
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