Importance

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Abstract

The assay reagent and kit of the present invention suppress non-specific binding of a labeled substance onto a solid phase, and can assay one or more species of antibodies or one or more species of antigens by means of a single reagent in a simple manner. The assay method involves reacting immunological ligands in a test sample with the assay reagent which contains a combination of components (A) and (B), thereby forming complexes, which complexes are captured onto the independently and separately present Solid phases (C), to assay the label contained in the complexes. Component (A): one or more species of immunological anti-ligand-nucleotide conjugates, in each of which nucleotides with a specific base sequence, independently selected depending on the species of an immunological ligand, are bound to an immunological anti-ligand having a specific immunological affinity to one of the different species of immunological ligands to be assayed; (B): labeled substances each having a specific affinity to one of the different species of immunological ligands to be assayed; Solid phase (C): solid phase-nucleotide conjugates wherein nucleotides, each having a base sequence complementarily binding to a nucleotide of the above component (A), are immobilized onto a water-insoluble support. Either an antigen or an antibody may serve as the aforementioned immunological ligand and immunological anti-ligand.

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First Claim