Compositions for the introduction of polyanionic materials into cells
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United States of America Patent
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Jul 28, 1998
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app pub date -
Sep 29, 1995
filing date -
Sep 30, 1994
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Abstract
The present invention provides compositions and methods which are useful for the introduction of polyanionic materials into cells. The compositions are mixtures of cationic compounds and neutral lipids which are typically formulated as liposomes. The cationic compounds are quaternary ammonium compounds wherein the nitrogen has two attached long chain alkyl groups, at least one of which is unsaturated. The methods for transfecting cells involve (a) contacting the polyanionic materials with the compositions above to form a polyanionic material-liposome complex, and (b) contacting the complex with the cells to be transfected.
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