Enzymatic oxidation of alcohols to aldehydes in a continuous reaction system
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United States of America Patent
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Jul 21, 1998
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N/A
app pub date -
Oct 9, 1996
filing date -
Dec 10, 1993
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Abstract
A process for the enzymatic oxidation of alcohols to aldehydes in a continuous reaction series is disclosed utilizing crude cellular methanol oxidase and catalase produced by an acceptable organism such as those selected from the genera of Pichia, Hansenula, Candida and Torulopsis. The process maintains a steady-state aldehyde concentration whereby enzyme activity is promoted. In particular, the process has two reactors operating in series continuously producing a fermenter effluent containing growing, intact yeast cells at a cell concentration of about 0.5 to about 3% by weight of the effluent by continuous culture of the cells on a methanol carbon source, continuously introducing the effluent from the first reactor to the second reactor, adding an alcohol feed at about 0.2-10% w/w and oxygen to the second reactor, maintaining a steady-state aldehyde concentration in the reaction mixture, the concentration of residual alcohol being about 0.1-9% w/w by controlling the rates of introduction of effluent and alcohol feed.
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