Method of detection of carcinogenic human papillomavirus
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United States of America Patent
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Jul 21, 1998
Grant Date -
N/A
app pub date -
Aug 25, 1989
filing date -
Feb 26, 1987
priority date (Note) -
Expired
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Abstract
Carcinogenic human papillomavirus types HPV16 and HPV18 are detected in a sample of cervical tissue. A selected characteristic DNA portion of the virus defined by oligonucleotide primers is amplified using a polymerase chain reaction involving successive heating and cooling steps, for example up to 250,000 copies. The presence/absence of the characteristic cloned DNA portion is detected by gel electrophoresis or using a labelled oligonucleotide probe.
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