Method for the isolation of lipoprotein allowing for the subsequent quantification of its mass and cholesterol content
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United States of America Patent
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Jul 21, 1998
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app pub date -
Jun 7, 1995
filing date -
Apr 27, 1990
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Abstract
A method is provided for preparing lipoprotein (a) from a volume of biological fluid that is substantially free of lipoproteins of another class. The method involves an ultracentrifugation step in which at least one fraction is recovered that contains Lp(a). This material is then reacted with immobilized ligand to remove non-Lp(a) interfering substances from the fraction, the Lp(a) remaining unbound. The non-reacted Lp(a) is subsequently obtained in a form that is suitable for use in the analysis of any of protein concentration, protein isoform determination or cholesterol assays. A method of identifying and measuring an amount of one or more isoforms of Lp(a) is further provided.
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