Expansion of bone marrow stromal cells
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United States of America Patent
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Jun 16, 1998
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app pub date -
Dec 29, 1995
filing date -
Dec 29, 1995
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Expired
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Abstract
The invention features a method for the selection and expansion of bone marrow stromal cells. The method includes the steps of obtaining bone marrow stromal cells; introducing the stromal cells into a vessel pre-coated on an inner surface with a gelatin, and containing a culture medium including an acidic fibroblast growth factor ('aFGF') polypeptide; and expanding the stromal cells in the culture medium under conditions and for a time sufficient to obtain an increased number of bone marrow stromal cells. The culture medium additionally can include heparin, and the vessel additionally can be precoated with fetal bovine serum.
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