Method for producing an asparaginyl endoprotease
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United States of America Patent
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Apr 14, 1998
Issued Date -
N/A
app pub date -
Jun 7, 1995
filing date -
Jul 22, 1992
priority date (Note) -
Expired
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Abstract
A method is provided for preparing an asparaginyl endoproteinase from the seeds of soybean, ginkgo and rice which have been collected between an early growing stage and ripening. The method comprises the steps of dialyzing an extract of the seeds against an acidic buffer of pH 4.0 to 6.0, ammonium sulfate precipitation, hydrophobic chromatography and gel filtration. The resulting asparaginyl endoproteinase cleaves glycinin between the C-terminal amino acid residue of the acidic subunit region, Asn, and the N-terminal amino acid residue of the basic subunit region, Gly or Asn.
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