Peptides including amino acid sequences selected from lipoprotein (a) and apolipoprotein (a), antibodies recognizing these amino acid sequences, and methods of determination using these antibodies
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United States of America Patent
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Mar 31, 1998
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N/A
app pub date -
Apr 14, 1994
filing date -
Aug 14, 1992
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Expired
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Abstract
Amino acid sequences having specificity as lipoprotein (a) and low homology with LDL and plasminogen are selected from the amino acid sequence of lipoprotein (a), and antibodies to lipoprotein (a) which recognize these amino acid sequences specifically are obtained. At least one of these antibodies is used in immunological techniques for the determination of lipoprotein (a). Moreover, an amino acid sequence having specificity as apolipoprotein (a) and not having antigenicity as lipoprotein (a) or plasminogen is selected from the amino acid sequence of apolipoprotein (a), and antibodies to apolipoprotein (a) which recognize this amino acid sequence specifically are obtained. At least one of these antibodies is used in immunological techniques for the determination of apolipoprotein (a). As compared with prior art methods, antibodies to lipoprotein (a) and apolipoprotein (a) can be obtained according to a simplified procedure, and their use makes it possible to determine lipoprotein (a) and apolipoprotein (a) accurately.
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