Method of colorimetric analysis of malonic dialdehyde and 4-hydroxy-2-enaldehydes as indexes of lipid peroxidation, kits for carrying out said method, substituted indoles for use in said method and their preparation

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United States of America Patent

PATENT NO 5726063
SERIAL NO

08702197

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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A colorimetric assay of enaldehydes or of malonic dialdehyde as lipid peroxidation indices in an aqueous medium including a) addition to the medium of a reagent of a compound of general formula I and their optional addition salts with organic or inorganic bases or with organic or inorganic acids. ##STR1## in which formula: A and C, which may be identical or different, each represent H, ##STR2## wherein A and C cannot simultaneously represent H, with: R.sup.1 =H; alkyl; aralkyl; aryl substituted on the aryl ring; alkyl sulfonate Y.sup.+ ; alkyl phosphonate, Y.sup.+ ; or alkyl carboxylate, Y.sup.+ ; R.sup.2 =H; --OR.sup.4 ; F; Cl; Br; I; --NO.sub.2 ; SO.sub.3.sup.31 Y.sup.+ ; --CN; --COOR.sup.4 ; or --CONR.sup.5 R.sup.6 ; R.sup.3 =H; --OR.sup.4 ; --NR.sup.5 R.sup.6 ; --SR.sup.4 ; F; Cl; Br; I; --NO.sub.2 ; --SO.sub.3 --Y.sup.+ ; --CN; --COR.sup.5 ; --COOR.sup.4 ; or --CONR.sup.5 R.sup.6 ; R.sup.4 =H; alkyl; aralkyl; or aryl substituted on the aryl ring; R.sup.5 =H; alkyl; aralkyl; or aryl substituted on the aryl ring; R.sup.6 =H; aryl, aralkyl; or aryl substituted on the aryl ring; Y.sup.+ =cation of an organic or inorganic base; ##STR3## wherein alkyl represents a linear or branched group comprising 1 to 6 carbon; aryl is substituted on the aryl ring with one or more groups which may be identical or different selected from C.sub.1-6 alkyl, alkoxy, hydroxy, amino and carboxyl; b) acidification of the medium; and c) incubation of the acidified medium. Requisites or kits for the implementation of the process are also disclosed.

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Patent Owner(s)

  • OXIS ISLE OF MAN, LIMITED

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Chaudiere, Jean Saint Maur Des Fosses, FR 11 49
Erdelmeier, Irene Paris, FR 16 64
Gerard-Monnier, Dominique Vitry Sur Seine, FR 1 10
Yadan, Jean-Claude Paris, FR 22 167

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