Synthetic gene for D4 dopamine receptors
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United States of America Patent
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Feb 24, 1998
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N/A
app pub date -
May 3, 1995
filing date -
Jul 1, 1993
priority date (Note) -
Expired
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Abstract
A modified gene coding for the human D4 dopamine receptor has been synthesized by chemo-enzymatic methods. The nucleotide sequence of the D4 dopamine receptor gene was changed to reduce the G+C content and to eliminate intronic sequences, while maintaining the published amino acid sequence. Using gene splicing by overlap extension and PCR amplification of long oligonucleotides (>200 bases), 3 synthetic fragments of about 400 base pairs each were amplified, from which the full length gene was assembled. Stable expression of this gene has been achieved in CHO-K1 cells, using an inducible expression system, and in HEK293 cells.
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