Cloning and expression of T5 DNA polymerase reduced in 3'-to-5' exonuclease activity
Number of patents in Portfolio can not be more than 2000
United States of America Patent
Stats
-
Feb 10, 1998
Grant Date -
N/A
app pub date -
Jul 29, 1996
filing date -
Aug 10, 1989
priority date (Note) -
In Force
status (Latency Note)
Importance
Abstract
The present invention discloses a recombinant DNA molecule having a structural gene encoding a processive, thioredoxin-independent DNA polymerase that is substantially reduced in processive 3'-to-5' DNA exonuclease activity, a promoter, and an origin of replication. A method for producing this enzyme is also disclosed, as is the protein produced by this process. This invention is exemplified by expression of exonuclease.sup.- T5 DNA polymerase in E. coli.
First Claim
Family
International Classification(s)
Cited Art Landscape
Patent Citation Ranking
Recipient Email Address
Recipient Email Address
Comment
Recipient Email Address
Add to Portfolio(s)
To add this patent to one, or more, of your portfolios, simply click the add button.
This Patent is in these Portfolios:
Add to additional portfolios:
Last Refreshed On:
Changes done successfully