Increased expression of a gene by a second transferred mammary gland specific sequence transgenic
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United States of America Patent
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Feb 3, 1998
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N/A
app pub date -
Jun 6, 1995
filing date -
Dec 24, 1990
priority date (Note) -
Expired
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Abstract
Transgenic animals capable of expressing a desired protein can be prepared by co-introducing into an egg or embryo cell of an animal, a first sequence, which encodes the desired protein, and a second, more efficiently expressed, DNA sequence. Expression efficiency is thereby conferred on the first sequence, leading to improved yield or targeting, or both. Co-introduction may be achieved by co-injecting a mixture of the two DNA sequences into a fertilized egg, in the case of an animal. The invention can be used to enhance the efficiency of expression of desired proteins, such as those having pharmaceutical activity in the mammary gland of a female transgenic animal.
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