Method and apparatus for liquid chromatography
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United States of America Patent
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Feb 3, 1998
Grant Date -
N/A
app pub date -
Sep 24, 1996
filing date -
Apr 26, 1993
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Abstract
In a method of filling a liquid chromatographic column with particulate separation medium, a liquid suspension of the particulate separation medium is introduced into one end of the column, wherein the column is closed at one end with a first filter, so that the particles will be retained by the filter while the liquid passes therethrough. After terminating the filling process, the other end of the column is closed with a second filter. According to the invention, the particle suspension is delivered via an inlet element which can be moved axially in relation to the end of the column an in which the second filter in mounted. During the filling process, the inlet element and the filter are held in a position in which the particle suspension is able to pass from the upper side of the filter and past its side-edge and into the interior of the column. After completion of the filling process, the inlet element is moved towards the end of the column, while maintaining liquid pressure, to a position in which by-passage of the filter is prevented, whereafter the inlet element is fixed in this position. The invention also relates to a column for carrying out the method.
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