Detection of mutation by resolvase cleavage
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United States of America Patent
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Dec 16, 1997
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N/A
app pub date -
Sep 16, 1996
filing date -
Apr 25, 1994
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Abstract
Methods are disclosed for detecting one or more mutations in an isolated test nucleic acid by forming a heteroduplex with a homologous control DNA and contacting the heteroduplex with a resolvase capable of recognizing at least one single base pair mismatch within the heteroduplex. In preferred embodiments of the invention, the resolvase is bacteriophage T4 endonuclease VII.
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