Purification process for hirudin using affinity chromatography
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United States of America Patent
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Dec 16, 1997
Grant Date -
N/A
app pub date -
Sep 6, 1995
filing date -
Sep 7, 1994
priority date (Note) -
Expired
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Abstract
Disclosed herein is a process for purifying hirudin from a solution containing hirudin and other substances, characterized in that the process comprises a step of subjecting the solution to a metal ion affinity chromatography wherein copper ion (Cu.sup.++) is used as a metal ion and a phosphate buffer is used as an eluent.
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