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Abstract

An improved method for producing natamycin by fermentation is disclosed. In the method, which comprises, in order, the steps of: (a) introducing an inoculum of a natamycin producing Streptomyces species to a fermentation medium to produce a fermentation broth comprising the fermentation medium and inoculum; and (b) producing natamycin by fermentation in the fermentation broth; the improvement comprising: (1) providing at least 15 g/L of a protein nitrogen source to the fermentation medium; wherein the protein nitrogen source comprises a non-yeast protein component and a yeast protein component, and the ratio of non-yeast nitrogen component to yeast protein component being from about 3:1 to 9:1, based on the protein content of the source; (2) providing a carbon source to the fermentation medium at from about 80 g/L to 250 g/L; wherein the carbon source addition is carried out during step (b) so as to maintain a concentration of carbon source of from about 5 g/L to 30 g/L; and (3) continuing the fermentation until the fermentation broth comprises at least 5 g/L natamycin.

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First Claim