Methods for enhanced production of tissue plasminogen activator in cell culture using alkanoic acids or salts thereof
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United States of America Patent
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Oct 28, 1997
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app pub date -
Dec 14, 1994
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Mar 14, 1986
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Abstract
Methods for enhancing the production of tissue plasminogen activator (TPA) in cell culture are disclosed. The methods involve culturing TPA-producing cells in growth media supplemented with an alkanoic acid or salt thereof at a concentration which enhances TPA production. The most preferred methods utilize butyric acid or sodium butyrate at a concentration of between 0.5 mM and 2.5 mM.
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