Procedure of separation of proteins by column chromatography using silica gels coated by a lipid bilayer
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United States of America Patent
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Sep 23, 1997
Grant Date -
N/A
app pub date -
Nov 30, 1994
filing date -
May 26, 1992
priority date (Note) -
Expired
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Abstract
A chromatography column packing material and column chromatographic methods using a silica gel based column packing material are disclosed. The silica gel material is coated by a phospholipid bilayer containing at least two lipids, at least one having an electric charge. Proteins are separated using this column packing material without denaturing because of the lipid coating. The protein molecule binds to the lipid bilayer coated silica gel and is then eluted by raising the temperature.
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