Method for inhibiting chymopapain and papain enzyme activity with polysaccharides of animal origin
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United States of America Patent
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Sep 23, 1997
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Oct 19, 1995
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Oct 19, 1995
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Abstract
The activities of chymopapain or papain in enzyme mixtures are inhibited by the addition of glycogen, hyaluronic acid, or desulfated heparin. The process for isolating viable hepatocytes or pancreatic cells by treatment with papain or chymopapain is improved by terminating the treatment by inhibiting the reaction with these polysaccharides. Similarly, the process for removing undesired tissue by treatment with papain or chymopapain is improved by terminating the treatment by inhibiting the reaction with these polysaccharides.
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