Modifying nucleotide analogues
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United States of America Patent
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Sep 23, 1997
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N/A
app pub date -
Mar 7, 1995
filing date -
Mar 8, 1994
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Expired
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Abstract
Fluorescently labelled dideoxynucleoside triphosphates are widely used as chain-terminators in Sanger dideoxy sequencing operations. But these unincorporated dye-terminators migrate in an electrophoresis gel and obscure the desired sequence ladder. This invention provides a method and a kit for modifying the unincorporated dye-terminators, e.g. by removal of a 5'-triphosphate group by chemical or enzymatic means e.g. by use of a phosphatase enzyme.
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