Automated analysis equipment and assay method for detecting cell surface protein and/or cytoplasmic receptor function using same
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United States of America Patent
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Sep 23, 1997
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N/A
app pub date -
Jun 20, 1994
filing date -
Dec 20, 1991
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Abstract
Automated measurement apparatus and methods for automated drug screening and for studying ion channel and cell surface receptor activity are provided. The apparatus is designed to initiate and measure rapid or transient events, such as cell receptor and/or ion channel activity. The apparatus can effect measurements of transient reactions in or more samples in a multi-well container, initiate the reaction with reagent addition and measure a resulting attribute of the sample for a period of time. The apparatus is capable of substantially continuously measuring and recording data corresponding to the measured attribute before, during and after initiation of the reaction so that a time course of the rapid or transient event is determined. Automated drug screening methods and methods for studying receptor and ion channel activity using the apparatus are also provided. The drug screening assays identify compounds that activate, inhibit or potentiate cellular ion channel or receptor activity.
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