Preparation of heterodimeric PDGF-AB using a bicistronic vector system in mammalian cells
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United States of America Patent
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Sep 9, 1997
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Apr 24, 1995
filing date -
Aug 27, 1992
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Abstract
The recombinant production of PDGF-AB in mammalian cells is disclosed by means of a bicistronic vector system in which an IRES sequence is located between the first and second cistrons and in which the B chain coding gene is located in the first cistron. The disclosed expression unit allows the equimolor expression of the A and B polypeptide chains.
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