RNA fingerprinting to determine RNA population differences
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United States of America Patent
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Sep 9, 1997
Grant Date -
N/A
app pub date -
Jun 9, 1995
filing date -
May 27, 1992
priority date (Note) -
Expired
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Abstract
A method of producing an RNA fingerprint of a poly(A).sup.+ RNA preparation. At least one oligonucleotide primer and a supply of nucleotides of which one is a terminator nucleotide, are added to the poly(A).sup.+ RNA preparation or a single-stranded cDNA preparation derived from it. cDNA products are synthesized from the RNA, or DNA products from the cDNA and the products are separated to produce an RNA fingerprint.
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