Method and kits for detection of fragile X specific, GC-rich DNA sequences
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United States of America Patent
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Aug 19, 1997
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Jun 7, 1995
filing date -
Jan 28, 1992
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Abstract
A method is provided for amplifying and detecting specific GC-rich nucleic acid sequences contained in a nucleic acid or in a mixture of nucleic acids, which includes treating a separate nucleic acid containing the specific sequence with a molar excess of primers and a polymerase and extending the primers in the presence of dATP, dCTP, TTP, and an analogue of dGTP. In one application of the present invention, individuals who are carriers for, or afflicted by, the fragile X syndrome are detected.
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