Column agglutination assay and device using biphasic centrifugation
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United States of America Patent
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Jul 22, 1997
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app pub date -
Apr 16, 1996
filing date -
Feb 26, 1993
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Abstract
The present invention provides a method and device for detecting the presence of binding ligands, especially blood group antigens or antibodies thereto, which utilize a matrix of substantially noncompressable microparticles, which matrix provides superior performance in allowing movement of nonagglutinated reactants, especially red blood cells, while constraining, preferably in a so-called 'band formation' agglutinated reactants, especially red blood cells. The device of the invention further comprises a chamber the transverse cross-sectional shape thereof being an ellipse, or preferably a flattened ellipse with two sides parallel to the longitudinal axis.
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