Method for measuring the activity of angiotensin converting enzyme in biological samples
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United States of America Patent
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Jun 3, 1997
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N/A
app pub date -
Jul 5, 1994
filing date -
Feb 1, 1991
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Expired
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Abstract
The activity of angiotensin converting enzyme (ACE) is measured in biological samples as body fluids. ACE-activity is estimated in minimally diluted specimens, using the natural substrate angiotensin I at close to physiological concentration. Femtomoles of generated angiotensin II are trapped by specific high affinity monoclonal antibodies and thus protected from degradation by angiotensinases during the incubation step. The same antibodies are subsequently used for quantitation by radioimmunoassay.
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