Method for the isolation and purification of vitamin K-dependent proteins
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United States of America Patent
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May 27, 1997
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Feb 27, 1995
filing date -
Feb 28, 1994
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Abstract
A method for the separation of vitamin K-dependent proteins from non-vitamin K-dependent accompanying proteins is described wherein the method is characterized in that at least anion exchange chromatography and optionally affinity chromatography is carried out as well. The method is suitable especially for the purification of Factor II, VII, IX, X as well as Protein S, Protein C and Protein Z. With the aid of the method according to the invention a vitamin K-dependent protein is obtained which is present at a purity of 95%.
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