Factor IX chromogenic assay
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United States of America Patent
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May 6, 1997
Issued Date -
N/A
app pub date -
Oct 13, 1994
filing date -
Aug 17, 1989
priority date (Note) -
Expired
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Abstract
A chromogenic assay for determination of blood coagulation Factor IX (christmas factor) utilizes Factor Xa formed by the conversion of Factor X by activated Factor IX and Factor VIII to cleave a chromogenic substrate. The sample is combined into a mixture with Factor XIa in the presence of Factor IIa (thrombin), incubated, combined with Factor X and Factor VIII, and incubated. A thrombin inhibitor, and an indicator agent which reacts with Factor Xa, are added and the resulting signal measured and correlated to the level of Factor IX in the sample.
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