Efficient gene probe conjugations by an unconventional mixed anhydride method
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United States of America Patent
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Apr 22, 1997
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N/A
app pub date -
Dec 19, 1994
filing date -
Oct 16, 1991
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Expired
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Abstract
A novel method for conjugating haptens, ligands or luminescent labels to polynucleotides has been developed. This method involves the formation of a mixed anhydride, followed by reaction of the mixed anhydride intermediate with a nucleophilic group-containing polynucleotide in a DMF/water solvent system. The resulting compound finds usefulness especially in the gene probe area.
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