DNA base sequence containing regions involved in the production and secretion of a protein, recombinant DNA including the whole or a part of the DNA base sequence, and method of producing proteins by use of the recombinant DNA
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United States of America Patent
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Mar 18, 1997
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Dec 20, 1994
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Dec 28, 1983
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Abstract
A neutral protease gene of Bacillus amyloliquefaciens is cloned, which gene comprises the promoter region, the ribosome binding region, the region involved in the secretion of the neutral protease, the region consisting of the structural gene for the neutral protease, and the terminator region. Each of the regions is useful as a material for construction of a recombinant DNA used for the production of proteins by culturing a host bacterium transformed with the recombinant DNA. For example, the extracellular production of neutral protease in a large amount can be accomplished by culturing B. subtilis transformed with a recombinant DNA comprising pUB110 and the neutral protease gene.
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