Purified Thermococcus barossii DNA polymerase
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United States of America Patent
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Feb 11, 1997
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Jan 18, 1995
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Jan 18, 1995
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Abstract
A preparation of a purified DNA polymerase substantially identical to native DNA polymerase isolated from Thermococcus barossii is disclosed. In a preferable form of the present invention, the DNA polymerase is approximately 68 kDa in size, retains at least 90% activity after 8 hours of incubation at 95.degree. C. and retains between 60% and 80% proof-reading ability when catalyzing a polymerization reaction with a primer with a 3 base pair mismatch.
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