Method for detecting target nucleic acid in specimen
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United States of America Patent
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Feb 11, 1997
Issued Date -
N/A
app pub date -
Sep 16, 1992
filing date -
Dec 25, 1987
priority date (Note) -
Expired
status (Latency Note)
Importance
Abstract
An intended nucleic acid is determined in a sample by a method in which at least one of the nucleic acid strands of the intended nucleic acid is subjected to hybridization with a primer and to chain-extension reaction over the primer to form a synthesized nucleic acid which is complementary to and in hybridization with the strand; a copy of the intended nucleic acid is obtained, which copy is (a) the nucleic acid of the double-stranded double produced by the chain-extension reaction, or (b) the synthesized nucleic acid freed from the double-stranded nucleic acid, or (c) a double stranded nucleic acid form from a pair of the synthesized nucleic acids complementary with each other; and it is determined whether the copy is present in the sample thereby to know whether the intended nucleic acid is in present in the sample.
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- 19 Citation Count
- C07H Class
- 77.85 % this patent is cited more than
- 28 Age