Purification of hapten-carrier generated antibodies
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United States of America Patent
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Dec 31, 1996
Issued Date -
N/A
app pub date -
Sep 6, 1994
filing date -
Jul 23, 1992
priority date (Note) -
Expired
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Abstract
In the commonly used method to obtain antibodies to small molecules, a combination of a highly antigenic carrier, such as bovine serum albumin, and the small molecule is injected into a host animal. The recovered crude serum or plasma then contains, in addition to the desired small molecule antibodies, much larger amounts of carrier-induced antibodies. These unwanted antibodies are efficiently removed from the crude serum or plasma, by contacting the crude serum or plasma with the carrier material in an immobilized high surface area form. Rapid and efficient anticarrier antibody removal results, with minimal loss of both desired antibody, and desired antibody activity.
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