Assay method based upon refractive index changes at a solid optical surface
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United States of America Patent
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Nov 12, 1996
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N/A
app pub date -
Feb 18, 1994
filing date -
Aug 20, 1991
priority date (Note) -
Expired
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Abstract
A method of assaying for an analyte in a fluid sample comprises detecting the presence of the analyte by determining the resulting change in refractive index at a solid optical surface in contact with the sample, which change is caused by the analyte involving or influencing the binding or release of a refractive index-enhancing species to or from, respectively, the optical surface. Determination is performed with light having a wavelength at or near the maximum of the negative derivative of the absorptivity with respect to wavelength of the refractive index-enhancing species to obtain maximum sensitivity.
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