Extracellular aminopeptidase isolated from Streptococcus thermonitrificans culture
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United States of America Patent
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Oct 29, 1996
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app pub date -
Jun 17, 1994
filing date -
Jun 17, 1993
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Abstract
An extracellular leucine aminopeptidase was isolated from the culture medium of Streptomyces therrnonitrificans which is a Zn.sup.2+ -dependent metalloenzyme which comprises an amino acid sequence Lys-Phe-Ser-Lys-Lys-Phe-Asn-Glu (SEQ ID NO: 3) at the N-terminal thereof, and amino acid sequences of Glu-Pro-Gly-Thr-Gly-Ala-Leu-Glu-Pro (SEQ ID NO: 4) and Asn-Pro-Asp-Ile-Val-Tyr (SEQ ID NO: 5) at other regions thereof. The aminopeptidase has a pH optimum of 7.5 to 9.0, a temperature optimum of 30.degree. to 50.degree. C., and an apparent molecular weight as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis under reducing conditions of 41 to 45 kDa, and as determined by gel filtration under native state conditions of 36 to 40 kDa.
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