Method and kit for enzymatically determining the pH of a specimen
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United States of America Patent
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Oct 22, 1996
Grant Date -
N/A
app pub date -
Mar 6, 1995
filing date -
Mar 6, 1995
priority date (Note) -
Expired
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Abstract
The present invention concerns a method of enzymatically determining the pH of a specimen (e.g., a solution or a biological fluid) and a kit for conducting the method. The present method involves mixing (1) a specimen with (2) an enzyme and (3) one or more substrates for the enzyme in a buffered solution having a pH effective to provide a direct proportional relationship between the activity of the enzyme and the pH of the specimen; determining the activity of the enzyme; and correlating the activity of the enzyme to the pH of the specimen. Each of the sample, the enzyme, the substrate and the buffered solution is present in an amount effective to provide the direct proportional relationship between the activity of the enzyme and the pH of the specimen. The present kit contains an enzyme, one or more substrates for the enzyme present in an amount effective to determine the enzyme activity, and a buffered solution having a pH effective to provide a direct proportional relationship between the activity of the enzyme and the pH of the specimen. The present invention is particularly applicable to detecting adulteration of a urine specimen.
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