Measurement of color reactions by monitoring a change of fluorescence
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United States of America Patent
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Oct 15, 1996
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app pub date -
Dec 27, 1995
filing date -
Aug 31, 1988
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Abstract
The present invention relates to a method to determine the concentration of an unknown substance in a colorimetric reaction using a fluorometric reader. The change in color can be monitored by observing the change of fluorescence of a known amount of a suitable fluorophore in the reaction chamber. Under appropriate conditions the absorption spectrum of the chromophore overlaps with the emission spectrum of the fluorophore thereby allowing the change in fluorescence to be proportional to the intensity of color in the reaction and thus, proportional to the quantity of the substance of interest. Specifically, 8-methoxypyrene tri-sulfonic acid is used as the fluorophore.
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