Detection reagent, article, and immunoassay method
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United States of America Patent
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Oct 1, 1996
Grant Date -
N/A
app pub date -
Jan 4, 1994
filing date -
Jan 4, 1994
priority date (Note) -
Expired
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Abstract
The binding site on an immunoglobulin for a protein is blocked by hydrophobically coupling the immunoglobulin to a blocking agent such as a label. This results in a detection reagent useful in a variety of test assays in which a protein and an immunoglobulin are advantageously used together, but separately. The reagent is useful in the simultaneous determination of IgG and one of IgA or IgM. Anti-IgA-IgG or anti-IgM-IgG is coupled to a hydrophobic label, particularly a pigment or dye, which label blocks the binding site on IgG for Protein A, and labeled Protein A is added.
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