Frog embryo and egg-derived tumor cell anti-proliferation protein
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United States of America Patent
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Sep 24, 1996
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N/A
app pub date -
Aug 1, 1994
filing date -
Apr 6, 1988
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Abstract
Rana pipiens eggs are subjected to fertilization and the embryos are grown to a predetermined stage of development. The embryos and unfertilized eggs are then subjected to mechanical processing in the presence of a weakly acidic buffer to produce an extract. The extract is subjected to ion-exchange chromatography and size-exclusion chromatography. The resulting pharmaceutical has activity against certain cancer cells. The amino acid sequence and composition of the pharmaceutical are disclosed.
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