Characterizing macromolecules interacting with at least three ligands on a sensor
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United States of America Patent
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Sep 10, 1996
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app pub date -
Dec 30, 1994
filing date -
Nov 10, 1988
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Abstract
A method of characterizing a macromolecule by studying its interactions with ligands comprises the determination of the mutual influence of ligand interactions by, after the macromolecule has interacted with at least one ligand, contacting the macromolecule with at least one additional ligand, either the macromolecule or the additional ligand or ligands having been bound to a sensor surface, determining interaction by detecting a consequential change in the physico-chemical properties of the sensor surface, and on the basis of the determined mutual dependence between the ligand interactions discriminating between epitopes of the macromolecule and mapping their relative positions.
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