Biological assay for microbial contamination
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United States of America Patent
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Aug 27, 1996
Grant Date -
N/A
app pub date -
May 27, 1994
filing date -
May 27, 1994
priority date (Note) -
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Abstract
Methods and kits for detecting the presence of and quantifying the concentration of viable microorganisms in a liquid sample are provided. Enzymes present in viable microorgansims reduce an indicator reagent to produce a visible color change. No titrations or dilutions of the sample are necessary. Variations of the test are described which can be used to detect a threshold level of viable microorganisms, to detect the presence or absence of viable microorganisms and to quantify the concentration of viable microorganisms in a liquid sample. The results are obtained rapidly with those for the detection available within thirty minutes and those for the quantification available within thirteen hours.
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