Two-step method of hypo-osmotic shock for reducing viability of bacteria
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United States of America Patent
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Aug 6, 1996
Grant Date -
N/A
app pub date -
Jun 29, 1994
filing date -
Jul 10, 1991
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Expired
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Abstract
A method for reducing the viability of bacterial cells is disclosed. Further, a two-stage process of hypo-osmotic shock is used by exposing cells to a first solution having a water activity (a.sub.w) of 0.997 or less. In addition after the first step the cells are further exposed to a solution of a higher a.sub.w that the first solution. The solutions are applied to the cells in the form of a spray or by immersion. The solutions are applied to the bacterial cells between 5 seconds and 30 minutes to obtain a reduction in the viability of the cells being treated of which are additionally treated with a solution of lysozyme contained in the solutions or to a cold shock treatment step. The cold shock treatment is applied by exposing the cells to a temperature in an aqueous liquid at 10.degree. C. or less.
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