Lactococcus promoters and signal sequences for heterologous gene expression in bacteria

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United States of America Patent

PATENT NO 5529908
SERIAL NO

08015582

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Abstract

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DNA sequences, derived from Lactococcus lactis subsp. lactis, are useful as promoters and promoter/secretion promoting signals for heterologous or homologous expression in Gram-positive bacteria. In another aspect, the invention relates to vectors, such as plasmids, comprising the sequences of the present invention, and to host cells transformed with such vectors. Yet additional aspects of the present invention are related to methods for producing desired heterologous or homologous peptides or proteins employing the sequences, vectors, or transformed hosts of the invention. By means of the invention, greatly improved heterologous and homologous expression and secretion may be achieved in E. coli and in Gram-positive bacteria such as B. subtilis, Lactococcus and Lactobacillus.

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Patent Owner(s)

Patent OwnerAddress
VALIO LTDTUOKKO PL 390 00101 HELSINKI FINLAND

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Koivula, Teija Helsinki, FI 1 10
Palva, Ilkka Helsinki, FI 6 29
Sibakov, Mervi Helsinki, FI 3 23
von, Wright Atte Kuopio, FI 3 12

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